Troubleshooting Unable to Start Real Time Analysis on the NovaSeq 6000

Users may experience an error regarding Real-Time Analysis (RTA) when attempting to start a run on the NovaSeq 6000. The error will read as follows:

Unable to start Real-Time Analysis. Restart the control software and set up a new run.

Typically this error is indicative of communications errors within the internal computer of the instrument and is resolvable with a complete power cycle of the system. For complete instructions on power cycling the NovaSeq 6000, please review the article How to Power Cycle the NovaSeq 6000 System.

If the error recurs, attempt to power cycle the instrument again. For this second power cycle, it is recommended to leave the instrument completely powered off for a minimum of 5 minutes.

If after a slow power cycle RTA continues to fail, contact Illumina Technical Support.

For any feedback or questions regarding this article (Illumina Knowledge Article #8431), contact Illumina Technical Support [e-mail].