# Introducing the NextSeq 1000/2000 P1 Reagent kit

This bulletin outlines the features and specifications of the new P1 reagent kit for the NextSeq 1000 and NextSeq 2000 systems.

**What’s new:**

* Usage of the new NextSeq 1000/2000 P1 reagent kit is enabled by an upgrade to [NextSeq 1000/2000 Control Software Suite v1.4](https://support.illumina.com/sequencing/sequencing_instruments/nextseq-1000-2000/downloads.html). This upgrade is required to run the new kit and can be self-installed.
* The P1 reagent kit introduces a lower throughput option for NextSeq 1000/2000 users.
* Along with the previously available P2 and P3 kits, the P1 kit expands the scalability and flexibility of the NextSeq 1000/2000 for more applications while allowing smaller batches, and projects to be run in < 20 hours.

**Table 1:** NextSeq 1000/2000 P1 specifications compared to P2 and P3 kits.

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-96727cd6ff2259ac5f13f55d67d2ec1a63105c46%2Fimage1.jpg?alt=media\&token=738af7b3-675a-486b-87af-f72a356319a5)

**Frequently asked questions:**

* **What sizes of P1 reagents are available?**\
  The NextSeq 1000/2000 P1 kit is only available in a 300-cycle size.
* **What NextSeq 1000/2000 Control Software version is required to run the P1 kit?**\
  Version 1.4 of the NextSeq 1000/2000 Control Software introduces compatibility with the P1 kit; older versions of the control software are not compatible.
* **Are the data from the P1 kit compatible with the onboard DRAGEN workflows?**\
  Yes, with NextSeq 1000/2000 Control Software v1.4, onboard DRAGEN versions 3.7 and 3.8 are both compatible with P1 data.
* **What is the expected run time for the P1 kit?**\
  A full 2 x 150 bp paired-end run takes around 19 hours to complete, with an average cycle time of about 2.8 minutes.
* **Are there any changes in loading concentration compared to other NextSeq 1000/2000 kits?**\
  The P1 kit uses the same loading concentration as the P2 and P3 kits.
* **How many cycles are in the P1 300-cycle kit?**\
  The P1 300-cycle reagent cartridge includes sufficient volume for an extra 38 cycles, allowing up to 338 cycles of sequencing.

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