# Library quantification and QC for Illumina TruSight RNA Pan Cancer libraries

**Which quality control method is recommended for the final libraries from TruSight RNA Pan-Cancer Library Prep?**

Assess the final library quality using either an Advanced Analytical Technologies Fragment Analyzer or Agilent Technologies 2100 Bioanalyzer.

**What is the typical library size distribution for TruSight RNA Pan-Cancer libraries?**

The size of the library distribution of DNA fragments ranges from approximately 200 bp - 1 kb, with a final average library size of approximately 250 - 300 bp. A larger fragment size is expected for good quality FFPE RNA (> 350 nt), while a smaller fragment size is expected for poor quality FFPE RNA.

**Which quantification method is recommended for TruSight RNA Pan-Cancer libraries?**

Illumina recommends qPCR.

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