# FAQ

Instrumentation > NextSeq 500/550 > FAQ

* [At which cycle of NextSeq 500/550 are run metrics calculated?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002584)
* [Can the NextSeq 500/550 v2.5 flow cell be used with the v2 Reagent cartridge and buffer cartridge?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002613)
* [Can the output folder for a NextSeq 500/550 run be saved to the local computer?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000005512)
* [Common Questions Related to NextSeq 500/550 v2.5 Kit](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002080)
* [Common questions regarding custom recipes for the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002618)
* [Do NextSeq 500/550 v2.5 runs have different specifications than v2.0 reagent runs?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002609)
* [How long does a NextSeq 500/550 system check take to complete?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002582)
* [How long does it take to purge all reagents in a NextSeq 500/550 run?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002581)
* [How many extra cycles are included in NextSeq 500/550 reagent cartridges?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002580)
* [How to set up a NextSeq 500/550 to upload to a BaseSpace Enterprise account using NCS v.4.0.1](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000005913)
* [Run setup modes for the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000005574)
* [What do the NextSeq 500/550 status bar colors mean?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000006206)
* [What is the difference between NextSeq PhiX Control Kit and PhiX Control v3?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002925)
* [What is the maximum read length supported in one direction on a NextSeq 500/550 run?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002588)
* [Will the NextSeq 500/550 Control Software prevent run setup with read lengths fewer than 25 cycles?](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002565)