# FAQ

Instrumentation > NextSeq 500/550 > FAQ

* [At which cycle of NextSeq 500/550 are run metrics calculated?](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002584.md)
* [Can the NextSeq 500/550 v2.5 flow cell be used with the v2 Reagent cartridge and buffer cartridge?](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002613.md)
* [Can the output folder for a NextSeq 500/550 run be saved to the local computer?](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000005512.md)
* [Common Questions Related to NextSeq 500/550 v2.5 Kit](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002080.md)
* [Common questions regarding custom recipes for the NextSeq 500/550](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002618.md)
* [Do NextSeq 500/550 v2.5 runs have different specifications than v2.0 reagent runs?](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002609.md)
* [How long does a NextSeq 500/550 system check take to complete?](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002582.md)
* [How long does it take to purge all reagents in a NextSeq 500/550 run?](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002581.md)
* [How many extra cycles are included in NextSeq 500/550 reagent cartridges?](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002580.md)
* [How to set up a NextSeq 500/550 to upload to a BaseSpace Enterprise account using NCS v.4.0.1](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000005913.md)
* [Run setup modes for the NextSeq 500/550](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000005574.md)
* [What do the NextSeq 500/550 status bar colors mean?](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000006206.md)
* [What is the difference between NextSeq PhiX Control Kit and PhiX Control v3?](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002925.md)
* [What is the maximum read length supported in one direction on a NextSeq 500/550 run?](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002588.md)
* [Will the NextSeq 500/550 Control Software prevent run setup with read lengths fewer than 25 cycles?](/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-faq-list/000002565.md)


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