Troubleshooting RheodyneValve error on NextSeq 500/550
Last updated
Last updated
© 2023 Illumina, Inc. All rights reserved. All trademarks are the property of Illumina, Inc. or their respective owners. Trademark information: illumina.com/company/legal.html. Privacy policy: illumina.com/company/legal/privacy.html
The following error on the NextSeq 500/550 indicates that there is an issue regarding the sippers controlled by the valves. The Rheodyne valve is not prone to failure, and this typically results from a transient communication error.
Error in Fluidics.FlowCellFluidics1.LanePump.IxPassThroughRheodyneValve1: Reinit while recovering from error””. Originally caused by” Timeout waiting 60000ms within EventToBclockingConverter.WaitForData().
Troubleshooting steps:
Power cycle the NextSeq 500/550.
Run the ‘Valve Response Test’ portion of the System Check.
Select Manage Instrument > select System Check.
Perform a manual quick wash for further testing.
If the error does not recur, the instrument is cleared for use.
If the error comes back during initialization, system check, or during the wash, contact Illumina Technical Support.
For further information, search for Knowledge Base articles How to Perform a NextSeq 500/550 Power Cycle and How to perform washes on the NextSeq 500/550.
For any feedback or questions regarding this article (Illumina Knowledge Article #1389), contact Illumina Technical Support techsupport@illumina.com.
