Troubleshooting RheodyneValve error on NextSeq 500/550

The following error on the NextSeq 500/550 indicates that there is an issue regarding the sippers controlled by the valves. The Rheodyne valve is not prone to failure, and this typically results from a transient communication error.

Error in Fluidics.FlowCellFluidics1.LanePump.IxPassThroughRheodyneValve1: Reinit while recovering from error””. Originally caused by” Timeout waiting 60000ms within EventToBclockingConverter.WaitForData().

Troubleshooting steps:

1. Power cycle the NextSeq 500/550.

2. Run the ‘Valve Response Test’ portion of the System Check.

   1. Select Manage Instrument > select System Check.

3. Perform a manual quick wash for further testing.

• If the error does not recur, the instrument is cleared for use.

• If the error comes back during initialization, system check, or during the wash, contact Illumina Technical Support.

For further information, search for Knowledge Base articles How to Perform a NextSeq 500/550 Power Cycle and How to perform washes on the NextSeq 500/550.

For any feedback or questions regarding this article (Illumina Knowledge Article #1389), contact Illumina Technical Support [e-mail].