Troubleshooting a Remote Server Returned An Error on NextSeq 500/550

The following error can occur at the start or during a run but will not cause the run to stop.

"Remote server returned an error: (426) Connection closed; transfer aborted"

This error is suspected to be caused by a temporary interruption in the network. Data transfer is expected to be resumed as soon as connection is restored.

Troubleshooting steps:

• If run has not stopped, allow instrument to proceed with sequencing and confirm all files are transferred successfully to the output location.

• If files were not transferred successfully to the output location, contact Illumina Technical Support for further assistance.

For any feedback or questions regarding this article (Illumina Knowledge Article #1812), contact Illumina Technical Support [e-mail].