How to troubleshoot Insufficient Memory errors during Pre Run Checks on the NovaSeq X Series Instruments

Background

Before starting a run, the Pre-Run Checks verify that the instrument is ready to perform a sequencing run and are split between the Dry and Wet checks. If the instrument does not have sufficient memory available to perform all of the necessary software actions during these Checks, it will present the following error:

Figure 1: Pre-Run Checks Errors - Insufficient memory to continue the execution of the program.

If the Pre-Run Checks are in the Dry Checks phase when the error occurs, the consumables will not be marked as used and are safe to be used in a follow up sequencing run.

If the Pre-Run Checks are in Wet Checks phase when the error occurs, the Flow Cell and Buffer Cartridge will be marked as used and new Flow cell and Buffer consumables will be required for a follow up sequencing run.

If this issue is observed, see the following steps to troubleshoot this issue.

Troubleshooting Actions

IMPORTANT NOTE: this issue is distinct from insufficient storage space and deleting sequencing runs will NOT resolve this issue. For issues with insufficient storage, see Troubleshooting Insufficient Storage Space Errors during Pre-Run Checks on the NovaSeq X Series.

1. Within the Control Software, select Return to Start to exit the Pre-Run Checks.

2. Power cycle the instrument according to Illumina guidelines listed in the How to power cycle the NovaSeq X Series Instruments Knowledge Article.

   1. For instruments running Control Software v1.2 and below, see section Restarting the Single Board Computer (SBC).

   2. For instruments running Control Software v1.2.2 and above, see section Power Cycling the NovaSeq X Series Instruments - For Control Software v1.2.2 and above.

3. Once the instrument has restarted and the Control Software is initialized, proceed with repeating the sequencing run setup.

If the issue persists, contact Illumina Tech Support for further assistance.

For any feedback or questions regarding this article (Illumina Knowledge Article #8466), contact Illumina Technical Support [e-mail].