At which cycle of NextSeq 500/550 are run metrics calculated?

The following diagram contains information on calculations made during the first 25 cycles during a NextSeq 500/550 run:

  • When is the final cluster density calculated?

  • When does template generation complete?

  • When is phasing/prephasing calculated?

  • When is clusters passing filter percentage determined?

  • When is base calling determined?

  • When is Q30 calculated (quality scoring)?

Last updated

© 2023 Illumina, Inc. All rights reserved. All trademarks are the property of Illumina, Inc. or their respective owners. Trademark information: illumina.com/company/legal.html. Privacy policy: illumina.com/company/legal/privacy.html