# At which cycle of NextSeq 500/550 are run metrics calculated?
The following diagram contains information on calculations made during the first 25 cycles during a NextSeq 500/550 run:
* When is the final cluster density calculated?
* When does template generation complete?
* When is phasing/prephasing calculated?
* When is clusters passing filter percentage determined?
* When is base calling determined?
* When is Q30 calculated (quality scoring)?
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