The following diagram contains information on calculations made during the first 25 cycles during a NextSeq 500/550 run:
When is the final cluster density calculated? Cycle 5
When does template generation complete? Cycle 5
When is phasing/prephasing calculated? Cycle 12
When is base calling determined? Cycle 12
When is clusters passing filter (PF) percentage determined? Cycle 25
When is Q30 calculated (quality scoring)? Cycle 25
Figure 1: Diagram illustrating sequencing run metric availability by cycle on the NextSeq 500/550.
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Last updated 6 days ago
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