At which cycle of NextSeq 500/550 are run metrics calculated?

The following diagram contains information on calculations made during the first 25 cycles during a NextSeq 500/550 run:

• When is the final cluster density calculated?

• When does template generation complete?

• When is phasing/prephasing calculated?

• When is clusters passing filter percentage determined?

• When is base calling determined?

• When is Q30 calculated (quality scoring)?

  

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