End of Life Schedule for NextSeq 1000/2000 SBS Standard Consumables

In effort to streamline Illumina's portfolio and ensure the highest quality products, Illumina obsolesced the following NextSeq 1000/2000 standard SBS reagent kits on December 12, 2025:

• NextSeq 1000/2000 P1 Reagents (100 Cycles)

• NextSeq 1000/2000 P1 Reagents (300 Cycles)*

• NextSeq 1000/2000 P1 Reagents (600 Cycles)*

• NextSeq 1000/2000 P2 Reagents (100 Cycles)*

• NextSeq 1000/2000 P2 Reagents (200 Cycles)*

• NextSeq 1000/2000 P2 Reagents (300 Cycles)*

• NextSeq 1000/2000 P2 Reagents (600 Cycles)

• NextSeq 2000 P3 Reagents (50 Cycles)

• NextSeq 2000 P3 Reagents (100 Cycles)*

• NextSeq 2000 P3 Reagents (200 Cycles)

• NextSeq 2000 P3 Reagents (300 Cycles)*

• NextSeq 1000/2000 Read and Index Primers

• NextSeq 1000/2000 Index Primer Kit

• NextSeq 1000/2000 Read Primer Kit

On June 3, 2024, Illumina introduced the NextSeq 1000/2000 XLEAP-SBS Reagent Kits to replace the standard SBS kits. Existing users were notified of this future availability in February 2024 via CCN2024-1513. NextSeq 1000/2000 standard SBS Reagent Kits will continue to be delivered with a guaranteed six-month shelf life up until the obsolescence date December 12, 2025 (*selected kits have extended availability with last order date pending and dependent on product availability). Available XLEAP reagents are available for purchase on Illumina's website here.

A concordance tech note comparing standard SBS and XLEAP SBS datasets on NextSeq 1000/2000 instruments can be found here.

The XLEAP-SBS Chemistry Quickstart guide, highlighting workflow changes can be found here.

For any feedback or questions regarding this article (Illumina Knowledge Article #9213), contact Illumina Technical Support [e-mail].