> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000001232.md).

# mRNA purification and rRNA removal information for TruSeq Stranded RNA workflows

This article addresses frequently asked questions on mRNA purification and rRNA removal for the TruSeq Stranded RNA Kit.

**Which molecules will be sequenced with the TruSeq Stranded mRNA Kit?**\
All poly-adenylated molecules will be isolated and sequenced. Note that there are species of non-coding RNA that are poly-adenylated as well.

**What is the difference between TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat and TruSeq Stranded Total RNA with Ribo-Zero Gold?**\
The Ribo-Zero workflow depletes samples of cytoplasmic ribosomal RNA. The Ribo-Zero Gold workflow depletes samples of both cytoplasmic and mitochondrial ribosomal RNA.

**How does the Ribo-Zero portion of the protocol work?**\
Ribo-Zero kits remove ribosomal RNA (rRNA) using a hybridization/bead capture procedure that selectively binds rRNA species using capture probes. The probe:rRNA hybrid is then captured by magnetic beads and removed using a magnet, leaving the desired rRNA-depleted RNA in solution.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #1232), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000001232%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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