# mRNA purification and rRNA removal information for TruSeq Stranded RNA workflows

**Which molecules will be sequenced with the TruSeq Stranded mRNA Kit?**\
All poly-adenylated molecules will be isolated and sequenced. Note that there are species of non-coding RNA that are poly-adenylated as well.

**What is the difference between TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat and TruSeq Stranded Total RNA with Ribo-Zero Gold?**\
The Ribo-Zero workflow depletes samples of cytoplasmic ribosomal RNA. The Ribo-Zero Gold workflow depletes samples of both cytoplasmic and mitochondrial ribosomal RNA.

**How does the Ribo-Zero portion of the protocol work?**\
Ribo-Zero kits remove ribosomal RNA (rRNA) using a hybridization/bead capture procedure that selectively binds rRNA species using capture probes. The probe:rRNA hybrid is then captured by magnetic beads and removed using a magnet, leaving the desired rRNA-depleted RNA in solution.

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