TruSeq Stranded RNA Kit FAQs: mRNA purification and rRNA removal

What molecules will be sequenced with the TruSeq Stranded mRNA Kit? All poly-adenylated molecules will be isolated and sequenced. Note that there are species of non-coding RNA that are poly-adenylated as well.

What's the difference between TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat and TruSeq Stranded Total RNA with Ribo-Zero Gold? The Ribo-Zero workflow depletes samples of cytoplasmic ribosomal RNA. The Ribo-Zero Gold workflow depletes samples of both cytoplasmic and mitochondrial ribosomal RNA.

How does the Ribo-Zero portion of the protocol work? Ribo-Zero kits remove ribosomal RNA (rRNA) using a hybridization/bead capture procedure that selectively binds rRNA species using biotinylated capture probes. The probe:rRNA hybrid is then captured by magnetic beads and removed using a magnet, leaving the desired rRNA-depleted RNA in solution.

For any feedback or questions regarding this article (Illumina Knowledge Article #1232), contact Illumina Technical Support [e-mail].