mRNA purification and rRNA removal information for TruSeq Stranded RNA workflows
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This article addresses frequently asked questions on mRNA purification and rRNA removal for the TruSeq Stranded RNA Kit.
Which molecules will be sequenced with the TruSeq Stranded mRNA Kit? All poly-adenylated molecules will be isolated and sequenced. Note that there are species of non-coding RNA that are poly-adenylated as well.
What is the difference between TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat and TruSeq Stranded Total RNA with Ribo-Zero Gold? The Ribo-Zero workflow depletes samples of cytoplasmic ribosomal RNA. The Ribo-Zero Gold workflow depletes samples of both cytoplasmic and mitochondrial ribosomal RNA.
How does the Ribo-Zero portion of the protocol work? Ribo-Zero kits remove ribosomal RNA (rRNA) using a hybridization/bead capture procedure that selectively binds rRNA species using capture probes. The probe:rRNA hybrid is then captured by magnetic beads and removed using a magnet, leaving the desired rRNA-depleted RNA in solution.
For any feedback or questions regarding this article (Illumina Knowledge Article #1232), contact Illumina Technical Support techsupport@illumina.com.
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