# How to Denature and Dilute Libraries on the NextSeq 500/550 Video This video goes through the necessary steps to denature and dilute libraries before running on the Nextseq 500/550. < {% embed url="" %} Video length: 3:24 min For further information, see [NextSeq 500/550 System Denature and Dilute Libraries Guide](https://support.illumina.com/downloads/nextseq-500-denaturing-diluting-libraries-15048776.html). \ \ \
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