# How to Denature and Dilute Libraries on the NextSeq 500/550 Video

This video goes through the necessary steps to denature and dilute libraries before running on the Nextseq 500/550.

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{% embed url="<https://www.youtube.com/watch?v=zM3Z41MyXUA&ab_channel=Illumina>" %}

Video length: 3:24 min

For further information, see [NextSeq 500/550 System Denature and Dilute Libraries Guide](https://support.illumina.com/downloads/nextseq-500-denaturing-diluting-libraries-15048776.html).

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