> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000002510.md).

# Index content for the TruSeq DNA and RNA Single Index kits, Sets A and B

TruSeq DNA and RNA single indexes must be purchased separately from library prep reagents\*. The following table lists the content of these index kits.

| TruSeq DNA Single Indexes Set A (20015960) | TruSeq RNA Single Indexes Set A (20020492) | TruSeq DNA Single Indexes Set B (20015961) | TruSeq RNA Single Indexes Set B (20020493) |
| ------------------------------------------ | ------------------------------------------ | ------------------------------------------ | ------------------------------------------ |
| AD002                                      | AR002                                      | AD001                                      | AR001                                      |
| AD004                                      | AR004                                      | AD003                                      | AR003                                      |
| AD005                                      | AR005                                      | AD008                                      | AR008                                      |
| AD006                                      | AR006                                      | AD009                                      | AR009                                      |
| AD007                                      | AR007                                      | AD010                                      | AR010                                      |
| AD0012                                     | AR012                                      | AD011                                      | AR011                                      |
| AD0013                                     | AR013                                      | AD020                                      | AR020                                      |
| AD0014                                     | AR014                                      | AD021                                      | AR021                                      |
| AD0015                                     | AR015                                      | AD022                                      | AR022                                      |
| AD0016                                     | AR016                                      | AD023                                      | AR023                                      |
| AD0018                                     | AR018                                      | AD025                                      | AR025                                      |
| AD0019                                     | AR019                                      | AD027                                      | AR027                                      |

\*Exceptions include TruSeq RNA Library Prep Kit v2 and TruSeq RNA Exome. TruSeq Single Indexes are bundled with these library preparation kits.

Additional information regarding the TruSeq Single Index kits:

* **Reactions per TruSeq DNA Single Indexes** **kit:** Each set contains 12 unique indexes that can be used to prepare a total of 24 libraries. Each index can be used twice.
* **Reactions per TruSeq RNA Single Indexes** **kit:** Each set contains 12 unique indexes that can be used to prepare a total of 48 libraries. Each index can be used four times.
* **Shipping and storage conditions:** Single index kits are shipped on dry ice and must be stored at -25°C to -15°C upon receipt.
* **Methylation status**: TruSeq single indexes are methylated and are suitable for bisulfite sequencing applications.
* **Index sequences:** Index sequences can be found in the [Illumina Adapter Sequences](https://support.illumina.com/downloads/illumina-adapter-sequences-document-1000000002694.html) document.
* **Pooling:** Low-plexity pooling guidelines can be found in the [Index Adapters Pooling Guide](https://support.illumina.com/downloads/index-adapters-pooling-guide-1000000041074.html).

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #2510), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000002510%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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