Index content for the TruSeq DNA and TruSeq RNA Single Index kits, Sets A and B

TruSeq DNA and RNA single indexes are purchased separately from library prep reagents*. The following table lists the content of these index kits.

*Exceptions include TruSeq RNA Library Prep Kit v2 and TruSeq RNA Exome. TruSeq Single Indexes are bundled with these library preparation kits.

Additional information regarding the TruSeq Single Index kits:

Reactions per TruSeq DNA Single Indexes kit: Each set contains 12 unique indexes that can be used to prepare a total of 24 libraries. Each index can be used twice.
Reactions per TruSeq RNA Single Indexes kit: Each set contains 12 unique indexes that can be used to prepare a total of 48 libraries. Each index can be used four times.
Shipping and storage conditions: Single index kits are shipped on dry ice and must be stored at -25°C to -15°C upon receipt.
Methylation status: TruSeq single indexes are methylated and are suitable for bisulfite sequencing applications.
Index sequences: Index sequences are found in the Illumina Adapter Sequences document.
Pooling: Low-plexity pooling guidelines are found in the Index Adapters Pooling Guide.

Last updated 6 months ago