Index content for the TruSeq DNA and TruSeq RNA Single Index kits, Sets A and B

TruSeq DNA and RNA single indexes are purchased separately from library prep reagents*. The following table lists the content of these index kits.

TruSeq DNA Single Indexes Set A (20015960)	TruSeq RNA Single Indexes Set A (20020492)	TruSeq DNA Single Indexes Set B (20015961)	TruSeq RNA Single Indexes Set B (20020493)
AD002	AR002	AD001	AR001
AD004	AR004	AD003	AR003
AD005	AR005	AD008	AR008
AD006	AR006	AD009	AR009
AD007	AR007	AD010	AR010
AD0012	AR012	AD011	AR011
AD0013	AR013	AD020	AR020
AD0014	AR014	AD021	AR021
AD0015	AR015	AD022	AR022
AD0016	AR016	AD023	AR023
AD0018	AR018	AD025	AR025
AD0019	AR019	AD027	AR027

*Exceptions include TruSeq RNA Library Prep Kit v2 and TruSeq RNA Exome. TruSeq Single Indexes are bundled with these library preparation kits.

Additional information regarding the TruSeq Single Index kits:

• Reactions per TruSeq DNA Single Indexes kit: Each set contains 12 unique indexes that can be used to prepare a total of 24 libraries. Each index can be used twice.

• Reactions per TruSeq RNA Single Indexes kit: Each set contains 12 unique indexes that can be used to prepare a total of 48 libraries. Each index can be used four times.

• Shipping and storage conditions: Single index kits are shipped on dry ice and must be stored at -25°C to -15°C upon receipt.

• Methylation status: TruSeq single indexes are methylated and are suitable for bisulfite sequencing applications.

• Index sequences: Index sequences are found in the Illumina Adapter Sequences document.

• Pooling: Low-plexity pooling guidelines are found in the Index Adapters Pooling Guide.

For any feedback or questions regarding this article (Illumina Knowledge Article #2510), contact Illumina Technical Support [e-mail].