How to clear hard drive space on the iSeq 100

A sequencing run on the iSeq 100 requires about 2.5 GB of local hard drive space. If space is low, a warning notification will be generated. Use the following steps to delete completed runs and clear space.

Note: Only delete runs using the iSeq 100 Control Software rather than manually through the operating system or File Explorer. Deleting runs manually can negatively impact the control software.

Note: Deleting runs via the Process Management workflow below will only remove them from the instrument's temporary folder (D:\Illumina\iSeq Runs[RunFolder])

1. From the control software menu, select Process Management.

   1. The Process Management screen appears with a list of runs saved to the local hard drive.

2. For the run to delete, select Delete Run.

   1. Deleting a run deletes the local run folder. The output folder, which is a copy of the run folder, is retained. If the output folder is designated as also being on the D:\ drive, it will have to be manually curated via the File Explorer. This will not negatively impact the control software like it would for manual curation of the temporary folder.

3. In the dialog box, select Yes, Delete Run to confirm deleting the run.

4. Repeat steps 2 and 3 to delete runs as needed.

5. When finished, close Process Management to return to the Home screen.

Note: Deleted files/folders cannot be recovered.

For any feedback or questions regarding this article (Illumina Knowledge Article #6672), contact Illumina Technical Support [e-mail].