> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-reference_material-list/000002260.md).

# Instructions for Installing the iSeq 100 Control Software

**Background**

The iSeq 100 instrument requires Control Software for setting up sequencing runs and normal instrument operation. If required, see the following instructions for installing the iSeq 100 Control Software.

*Installing the iSeq 100 Control Software*

1. Navigate to the [iSeq 100 Control Software Downloads](https://support.illumina.com/downloads/iseq-system-software-v31.html) page and download the iSeq 100 Control Software installer.
2. Once downloaded, log into the iSeq 100 as `sbsadmin` or another Windows Administrator account.
3. Unzip `iSeqSuiteInstaller.exe` to `C:\Illumina` directory on the iSeq 100 instrument.
   * **Note**: Installation from other folders will not work.
4. Double click/tap on the installer and follow the on-screen prompts to complete the installation.\
   ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-4eeeb4e6a294dd11552b548a5a5b3ced8caeec4c%2Fimage1.png?alt=media\&token=df4498e9-1f08-4698-8146-c0581cf8bb60)\
   ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-65249db3b8f8a2f99c00c5e5f03713859ae7ccd7%2Fimage2.png?alt=media\&token=f5bec907-3037-4f5e-bfb3-145d58ed0395)
5. For some software releases, there will be a registry prompt to re-enable Windows **Software Restriction Policy (SRP). Select Yes to re-enable SRP.**
   * If unable to do so, SRP can be re-enabled by navigating to `C:\Illumina\Security` and double-clicking on `Enable.reg.`

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-909b4205c619b4fe391bb4c8b96d0169d55fd7e7%2Fimage3.jpg?alt=media)

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                        |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #2260), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000002260%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


---

# Agent Instructions
This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com.

## Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter, and the optional `goal` query parameter:

```
GET https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-reference_material-list/000002260.md?ask=<question>&goal=<endgoal>
```

`ask` is the immediate question: it should be specific, self-contained, and written in natural language.
`goal` is optional and describes the broader end goal you are ultimately trying to accomplish on behalf of the user. GitBook uses it to tailor the answer towards what is most useful for that goal.

The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.