> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/instrumentation/general/instrumentation-general-reference_material-list/000008380.md).

# How to requeue an analysis on BaseSpace Sequence Hub when no changes to the Sample Sheet are needed

**Background**

Analyses for runs can be requeued through the BaseSpace Sequence Hub (BSSH) Web Interface the majority of the time with a v1 or v2 sample sheet. Instruments that typically use a v1 sample sheet include the iSeq 100, MiniSeq (updated to Control Software 2 and Windows 10), MiSeq, NextSeq 500/550 (updated to NextSeq 500/550 Control Software 4 and Windows 10), and NovaSeq 6000. Illumina recommends using the Local Run Manager software to create v1 sample sheets and BaseSpace Run Planning to create v2 sample sheets, as they guide users through the process and perform validation checks for errors.

If an analysis needs to be requeued using the same sample sheet, follow the instructions below.

**Important notes**

* Only the owner of the run can requeue an analysis. It is possible to [transfer ownership](https://help.basespace.illumina.com/manage-data/transfer-ownership) of a run, if necessary. A [shared run](https://help.basespace.illumina.com/articles/tutorials/share-data-using-getlink/) does not provide the necessary permissions needed to requeue analysis.
* There is a limit of five requeue attempts for users of BaseSpace Sequence Hub. After reaching the five requeue limit, users can use the free BCL Convert App to requeue the run even if using a v1 sample sheet. If assistance with additional requeues is needed, email [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support) and share the run ID and sample sheet. The run ID is the numerical value after: [https://basespace.illumina.com/runs/](https://basespace.illumina.com/runs/active) located in the web browser address bar in the BaseSpace Run page.
* If the five requeue limit has been reached and a change in the sample sheet is required, the requeue can be done following the steps described in the following Knowledge article [How to requeue FASTQ Generation using BCL Convert on BaseSpace](/software/general/software-general-faq-list/000006472.md).

**To requeue the analysis using the same sample sheet:**

1. After logging in to the BaseSpace account, select the **RUNS > Sequencing Runs** at the left side menu of the page.
2. Select the run to requeue.
3. Select the **hourglass** icon, then **REQUEUE** > **SAMPLE SHEET**.
4. The sample sheet may be pre-populated in the editing window. If not, select 'Load Original' to load the sample sheet.
5. Once the sample sheet has been validated, select the blue **Requeue** button.
   * If the **Requeue** button is not active, check for and resolve any validation errors.
6. The status on the run summary page will display "Analyzing" when the analysis is ongoing and will change to "Complete" when the requeued analysis is complete.

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-8e69c434c8f9d1aa91199068888e9e10f1684c5e%2Fimage1.jpg?alt=media\&token=6fe24abc-f431-4e69-b8c1-79dc73dd3afc)

If reformatting of the sample sheet is required prior to requeuing the analysis, see Knowledge article [How to edit a sample sheet and requeue an analysis in BaseSpace Sequence Hub](/software/general/software-general-reference_material-list/000001321.md)

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #8380), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000008380%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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