Types of small RNA and the need for a separate small RNA kit
Most common types of small RNA:
Abbreviation
Name
Length
Description
miRNA
microRNA
18-25 nt
Inhibit translation of mRNA.
siRNA
small interfering RNA
20-25 nt (longer has been reported)
Cleave mRNA
piRNA
Piwi-interacting RNA
24-31 nt
Silence transposons in the germline
Why is a separate small RNA kit required? The small RNAs are too small to be captured by the Total RNA and mRNA library preparation kits. Library fragments generated from such small RNAs are not faithfully retained during bead clean-up steps.
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