Pre run check failure due to "I/O error" on the NextSeq 1000/2000

The "I/O error" occurs during the DRY checks of the pre-run checks on the NextSeq 1000/2000.

To troubleshoot:

1. Power cycle instrument and re-attempt run setup with same consumables. Search for Knowledge Article: How to power cycle the NextSeq 1000/2000.

2. If error persists after power cycling, remove cartridge from the instrument and perform system checks. Search for Knowledge Article: System Checks for the NextSeq 1000/2000.

3. Collect the following files from the instrument:

   1. System check results (located in /usr/local/illumina/systemcheck)

   2. Run set up logs (located in /usr/local/Illumina/Runs/Setup/[Date_Time]).

4. Provide the collected files in Step 3 to Illumina Technical Support for evaluation and next steps.

For any feedback or questions regarding this article (Illumina Knowledge Article #5170), contact Illumina Technical Support [email protected].