# Pre run check failure due to "I/O error" on the NextSeq 1000/2000

The "I/O error" occurs during the DRY checks of the pre-run checks on the NextSeq 1000/2000.

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-46674ed0931d1a59243a40c5ca68d5c06644ee5c%2Fimage1.png?alt=media)

To troubleshoot:

1. Power cycle instrument and re-attempt run setup with same consumables. Search for Knowledge Article: **How to power cycle the NextSeq 1000/2000**.
2. If error persists after power cycling, remove cartridge from the instrument and perform system checks. Search for Knowledge Article: **System Checks for the NextSeq 1000/2000**.
3. Collect the following files from the instrument:
   1. System check results (located in /usr/local/illumina/systemcheck)
   2. Run set up logs (located in /usr/local/Illumina/Runs/Setup/\[Date\_Time]).
4. Provide the collected files in Step 3 to [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/) for evaluation and next steps.

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