Troubleshooting pre run check failure due to "Imaging test failed" on the NextSeq 1000/2000

Users may encounter the following error during pre run checks:

Imaging test failed. The imaging metric Worst bit error rate was out of spec.

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This error occurs during dry pre run checks, reagents have not yet been marked as used.

To troubleshoot:

1. Select "Retry" to re-attempt starting the run.

2. If error persists after retrying, Power Cycle the instrument and attempt to start the run again. See How to power cycle the NextSeq 1000/2000.

3. If error persists after power cycling instrument, collect the following files and contact Illumina Technical Support:

   1. Perform Bit Error Rate test of System Check, results are found within /usr/local/illumina/systemcheck. See System Checks for the NextSeq 1000/2000.

   2. Control Software logs within /usr/local/illumina/logs/next1k2k-control-software

   3. Run set up logs within /usr/local/illumina/runs/setup/

For any feedback or questions regarding this article (Illumina Knowledge Article #9854), contact Illumina Technical Support [email protected].