Troubleshooting pre run check failure due to "Imaging test failed" on the NextSeq 1000/2000

Users may encounter the following error during pre run checks:

Imaging test failed. The imaging metric Worst bit error rate was out of spec.

This error occurs during dry pre run checks, reagents have not yet been marked as used.

To troubleshoot:

1. Select "Retry" to re-attempt starting the run.

2. If error persists after retrying, Power Cycle the instrument and attempt to start the run again. See How to power cycle the NextSeq 1000/2000.

3. If error persists after power cycling instrument, collect the following files and contact Illumina Technical Support:

   1. Perform Bit Error Rate test of System Check, results are found within /usr/local/illumina/systemcheck. See System Checks for the NextSeq 1000/2000.

   2. Control Software logs within /usr/local/illumina/logs/next1k2k-control-software

   3. Run set up logs within /usr/local/illumina/runs/setup/

For any feedback or questions regarding this article (Illumina Knowledge Article #9854), contact Illumina Technical Support [e-mail].