# Troubleshooting pre run check failure due to "Imaging test failed" on the NextSeq 1000/2000

Users may encounter the following error during pre run checks:

**Imaging test failed. The imaging metric Worst bit error rate was out of spec.**

\*\* ![](/files/TSsBgssYew67F3fZsGyz)\
\*\*

This error occurs during dry pre run checks, reagents have not yet been marked as used.

**To troubleshoot**:

1. Select "Retry" to re-attempt starting the run.
2. If error persists after retrying, Power Cycle the instrument and attempt to start the run again. See [How to power cycle the NextSeq 1000/2000](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-reference_material-list/000003038#:~:text=To%20power%20cycle%20the%20instrument%3A%20From%20the%20control,side%20of%20the%20instrument%20until%20the%20lights%20fade.).
3. If error persists after power cycling instrument, collect the following files and contact Illumina Technical Support:
   1. Perform **Bit Error Rate test** of System Check, results are found within /usr/local/illumina/systemcheck. See [System Checks for the NextSeq 1000/2000](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-reference_material-list/000003457).
   2. **Control Software logs** within /usr/local/illumina/logs/next1k2k-control-software
   3. **Run** **set up logs** within /usr/local/illumina/runs/setup/

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                        |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #9854), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000009854%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


---

# Agent Instructions: Querying This Documentation

If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-troubleshooting-list/000009854.md?ask=<question>
```

The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.
