Library prep workflow frequently asked questions for the Illumina miRNA prep kit

How does this kit capture miRNA?

• RNAs with a 3′ hydroxyl group and a 5′ phosphate group (such as miRNA and piRNA) will be captured into the library. Generally speaking, any RNA molecule that is 50 bp or smaller, and has a 3′ hydroxyl and 5′ phosphate, will be robustly included in the library.

What happens when the 3’ or 5’ adapters are NOT diluted for low RNA input amounts or serum/plasma?

• Adapter dimers increase.

Can customers store the primer/adapter dilutions that are made in the protocol? If so, for how long and at what temperature?

• This is not recommended. It is best to make fresh dilutions for each batch/day.

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