Representative library QC traces for Illumina Single Cell 3’ RNA Prep

The final Illumina Single Cell 3' RNA Prep library distribution and average fragment size may vary based on sample type and experimental condition(s).

The following TapeStation-generated traces serve as representative examples of satisfactory library QC profiles (from satisfactory cDNA QC) generated during the "Check Library" step of the Illumina Single Cell 3' RNA Prep workflow. For more information, see the appropriate reference guide.

Human peripheral blood mononuclear cells (PBMCs)

Human HEK 293T & mouse NIH 3T3 cell line mixture

Mouse frozen tissue: unfixed brain nuclei

Mouse frozen tissue: DSP-methanol fixed brain nuclei

Mouse frozen tissue: unfixed lung nuclei

Refer to Assessing library QC in Illumina Single Cell 3’ RNA Prep and the Illumina Single Cell 3' RNA Prep Training Packet for further guidance and library QC best practices.

For any feedback or questions regarding this article (Illumina Knowledge Article #9528), contact Illumina Technical Support [e-mail].