Troubleshooting Pressure decay or Impedance test stopped with NaN results on the NovaSeq 6000

If Pressure Decay or Impedance test on the NovaSeq 6000 system check stops with NaN result:

• Check pressure sensor readings:

  • Close NovaSeq Control Software.

  • Open NovaSeq Service Software (NVSS).

  • Navigate to Integrated Chassis Assembly.

  • Navigate to the Sensors Tab and select the refresh icon for Fluidics.

  • If any sensor is >2.5psi, take a screenshot or picture of the result and collect NovaSeq Service Software Logs located under C:\ProgramData\Illumina\NovaSeq\NovaSeq Service Software\Logs and contact Illumina Technical Support for the next steps.

  • Note: Pressure sensors are not used during a run and, therefore, failing sensors will not impact a run performance. The instrument can continue sequencing until an Illumina Field Service Engineer (FSE) can replace the sensors.