Guidance for RNA isolated from PAXgene Blood RNA kit

Due to the presence of enzymatic inhibitors, RNA isolated using a PAXgene kit requires an additional cleanup step.

For total RNA isolated using a PAXgene Blood RNA kit or PAXgene Blood miRNA kit, do the following:

  1. Purify the total RNA using one of the following procedures:

    1. Modified RNeasy MinElute Cleanup Kit as described in Modified RNeasy MinElute Cleanup Kit on page 18 of the Globin-Zero Gold rRNA Removal Kit Reference Guide (Document# 15066013 v02).

  2. Elute in 12 ul RNase-free water

For any feedback or questions regarding this article (Illumina Knowledge Article #8483), contact Illumina Technical Support techsupport@illumina.com.

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