Reference Material

Library Preparation > General > Reference Material

• Adapter dimers的形成原因、影响及去除方法

• Amplicon Sequencing Introduction Support Webinar Video

• Bead handling best practices

• Bead types in Illumina library preparation kits

• Best practices for library quantification

• Best practices for manually normalizing library concentrations

• Best practices for using Sodium Hydroxide with Illumina sequencing systems

• Bubble products in sequencing libraries: causes, identification, and workflow recommendations

• Choosing a Library Loading Concentration for Illumina Sequencing Video

• Compatible and recommended Illumina library types for the NovaSeq 6000 sequencing platform

• Considerations when migrating non Illumina libraries between sequencing platforms

• DNA/RNA isolation considerations when using Illumina library prep kits

• Final library trace examples for the Illumina Library Prep Kits

• Fusion Genes kits for detection and lists of targeted genes

• How Can I Tell If I Sequenced Through the Insert? Part 1 Video

• How Can I Tell If I Sequenced Through the Insert? Part 2 Video

• How Do I Achieve Consistent Quantitation? Part 1 Video

• How Do I Achieve Consistent Quantitation? Part 2 Video

• How Do I Optimize Amplicon Sequencing Data? Part 1 Video

• How Do I Optimize Amplicon Sequencing Data? Part 2 Video

• How short inserts affect sequencing performance

• How to Plan Your First Sequencing Project Support Webinar Video

• How to Use the Illumina Library Prep Kit Selector Video

• How to find a Safety Data Sheet

• How to mitigate index hopping

• How to see more content and products relevant to your field of interest

• How to use Illumina DesignStudio for Custom Sequencing Panels Video

• How to use the Illumina Sequencing Coverage Calculator Video

• IDT for Illumina DNA/RNA Unique Dual Index Compatibility on the MiniSeq

• Illumina DNA Prep with Enrichment – Tagmentation: Best Practices and Troubleshooting Video

• Illumina adapter portfolio

• Illumina library prep kits and associated index kits

• Illumina 接头系列

• Illumina建库试剂盒和对应的index试剂盒

• Index content for the TruSeq DNA and TruSeq RNA Single Index kits, Sets A and B

• Indexed Sequencing Overview for Paired End Flow Cells

• Introduction to Illumina DesignStudio Support Webinar Video

• Is it possible to pool different library types in the same sequencing run?

• Library quantification and quality control quick reference guide

• Metagenomics Part I Introduction to Library Preparation and Sequencing Support Webinar Video

• NextSeq 1000/2000 Loading Optimization for XLEAP SBS kits

• Optimal Cluster Density Best Practices Video

• PhiX Control v3文库是什么，它在Illumina下一代测序中起到什么作用？

• Plate layout for AmpliSeq Unique Dual (UD) indexes

• Plate layout for TruSeq Combinatorial Dual (CD) indexes

• Plate layout for TruSeq Unique Dual (UD) indexes

• Plate layouts for IDT for Illumina DNA/RNA UD Index kits, Sets A, B, C, and D

• Recommended quality control of FFPE samples for Illumina FFPE supported library preparation kits

• Resources for getting started with Illumina Next Generation Sequencing (NGS)

• Sequencing FFPE Samples Introduction Support Webinar Video

• Sequencing Method Explorer：哪种二代测序（NGS）方法最适合我的研究？

• Sequencing primer compatibility and recommended library kits for the HiSeq 1000/2000 and 1500/2500

• Sequencing primer compatibility and recommended library kits for the NextSeq 1000/2000 platforms

• Sequencing primer compatibility and recommended library types for the HiSeq X and HiSeq 3000/4000

• Sequencing primer compatibility of Illumina libraries and kit types for NextSeq 500/550 and MiniSeq

• Sequencing primer compatibility of Illumina libraries and library types for the iSeq 100

• Sequencing primer compatibility of Illumina libraries and recommended library kit for the MiSeq

• Spiking custom primers into the Illumina sequencing primers

• TruSight RNA Fusion Panel product page and support page links

• TruSight RNA Pan Cancer Panel product page and support page links

• Understanding the differences between Illumina index chemistries and anchor function

• Understanding unique dual indexes (UDI) and associated library prep kits

• Using Sequencing Method Explorer to identify suitable NGS methods for research

• What is the PhiX Control v3 Library and what is its function in Illumina Next Generation Sequencing

• What sequences do I use for adapter trimming

• Why pooling libraries at the same concentration does not change the final pool concentration

• 了解唯一双标签（UDI）与相关建库试剂盒

• 将定制引物掺入到Illumina测序引物中

• 手动文库浓度标准化最佳操作

• 文库定量与质量控制简明指引

• 是否可以混合不同类型的文库并同时进行测序？

• 最小化 TruSeq Stranded Total RNA 文库中rRNA污染的最佳操作

• 末端接头剪切（adapter trimming）时需要用到的序列