FAQ

Library Preparation > DNA Library Prep > FAQ

• Analysis FAQ for Illumina DNA PCR Free libraries

• BLT PF handling for the Illumina DNA PCR Free workflow questions

• BLT PF usage FAQ for Illumina DNA PCR Free

• Bead clean up ratios used in the Illumina 16S demonstrated protocol

• Bead size selection FAQ for Illumina DNA PCR Free

• Can FFPE input be used with the Illumina DNA PCR Free library preparation kit?

• Consumables used in Illumina DNA PCR Free workflow

• Differences between Illumina DNA Prep (M) Tagmentation (Nextera DNA Flex), Nextera DNA, and Nextera XT kits

• Does the Flex Lysis Reagent Kit require the purchase of additional Illumina reagents?

• How does the Illumina/Nextera tagmentation enzyme make sure that both p5 and p7 adapters are added?

• How to quantify and what is the expected yield for Illumina DNA PCR Free libraries?

• Illumina DNA PCR Free custom sequencing primer FAQ

• Index selection and FAQ for Illumina DNA PCR Free

• Indexing FAQ for Illumina DNA PCR Free libraries

• Input quantity for Illumina DNA PCR Free libraries

• Input types FAQ for Illumina DNA PCR Free libraries

• Instrument, flow cell, and reagent requirement FAQ for Illumina DNA PCR Free libraries

• Is the Nextera TDE1 enzyme pre loaded with oligos?

• Library storage FAQ for Illumina DNA PCR Free libraries

• Product page and support page for Illumina DNA Prep, (M) Tagmentation

• Product page and support page links for Illumina DNA PCR Free

• Product page and support page links for Nextera XT

• Product page and support page links for TruSeq ChIP

• Qualification/QC FAQ for Illumina DNA PCR Free libraries

• Saliva collection for Illumina DNA PCR Free library preparation questions

• Saliva processing for Illumina DNA PCR Free library preparation workflow questions

• Sample Input for Illumina DNA Prep, (M) Tagmentation Library Preparation Kit

• Sample batching with the Illumina DNA PCR Free kit questions

• Samples per sequencing run and coverage FAQ for Illumina DNA PCR Free

• Sequencing run setup software considerations FAQ for Illumina DNA PCR Free libraries

• Single or double sided bead clean ups with Illumina DNA PCR Free.

• Size selection and clean up considerations for TruSeq DNA PCR Free library preparation

• Standard vs Low Input Workflow selection FAQ for Illumina DNA PCR Free prep

• Using dried blood spots as input into the Illumina DNA PCR Free library prep kit questions

• What is the Illumina Lysis Reagent Kit, and when should it be used?

• What loading concentration is recommended for Illumina DNA Prep on MiSeq v2 kits?

• When to quantify libraries and method of quantification for Illumina DNA PCR Free libraries

• Which Nextera Index Kits come in plate or tube format?

• Whole blood input requirements for Illumina DNA PCR Free