Optimal cluster density guidelines for the NextSeq 500/550
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The recommended raw cluster density for NextSeq 500/550 High-Output and Mid-Output runs is 170-220k/mm2 for well-balanced, base diverse libraries (eg, whole genome libraries, RNA-seq libraries, etc.).
For further information, search for Knowledge Base articles:
Cluster density guidelines for Illumina sequencing platforms.
How to achieve more consistent cluster density on Illumina Sequencing Platforms.
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