Steps if insufficient wash solution was used in a manual wash on the NextSeq 500/550 or MiniSeq
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If there is not enough wash solution in the wash tray during a manual wash, this can introduce air into the system which can negatively impact subsequent sequencing run performance.
To fix this, Illumina recommends performing 2-3 maintenance washes on the instrument to flush the fluidics lines and remove any air bubbles.
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