Information on controls used in TruSight Oncology 500 demo datasets

Background

Illumina provides demo datasets for the TruSight Oncology 500 (TSO500) assay and analysis in the Demo Data section of BaseSpace to allow customers to understand expected outputs and results. Each demo dataset contains commercially available control samples, with some also including internally-derived test samples. In the tables below demo datasets are listed along with the controls used in each.

TSO500 (FFPE, solid assay)

TruSight Oncology 500; Commerical controls (DNA + RNA) on NextSeq 550Project:https://basespace.illumina.com/s/RfIEI4oB2ePi

Sample ID	Sample Type	Vendor	Item
SeraCare0710_0097	DNA	SeraCare	Seraseq® Tri-Level Tumor Mutation DNA Mix v2
HorizonDx_HD827	DNA	HorizonDx	HD827: Mimix™ OncoSpan, gDNA Reference Standard
HorizonDx_HD753	DNA	HorizonDx	HD753: Mimix™ Structural Multiplex, FFPE Reference Standard
MilliporeSigma_87060101_1VL	DNA	MilliporeSigma	LoVo Cell Line human
ATCC_HCC_1395_CRL_2324D	DNA	ATCC	HCC1395 CRL-2324 ™
ATCC_A2058_CRL_11147D	DNA	ATCC	A2058 CRL-3601 ™
ATC_CCCL_225DQ	DNA	ATCC	HCT-15 Purified Genomic DNA, Quantitative (KRAS Mutant)
RNA_SeraCare_0710_0496	RNA	SeraCare	Seraseq® FFPE Tumor Fusion RNA v4 Reference Material
RNA_SeraCare_0710_1031	RNA	SeraCare	Seraseq® FFPE NTRK Fusion RNA Reference Material
RNA_SeraCare_0710_0137	RNA	SeraCare	Seraseq® FFPE WT (DNA/RNA) Reference Material
UHR_740000	RNA	Agilent	Universal Human Reference RNA

NovaSeq 6000Dx: TruSight Oncology 500 v2 Project: https://basespace.illumina.com/s/k5If7vXHZshI

Sample ID	Sample Type	Vendor	Item
HD795_rep1_dna	DNA	HorizonDx	HD795: Mimix™ BRCA Somatic Multiplex I, FFPE Reference Standard

TSO500 ctDNA (liquid assay)

Control	Demo data
Seraseq® ctDNA Complete Mutation Mix	TruSight Oncology 500 ctDNA v2; SeraSeq ctDNA Complete Mutation Mix variants at 0.2% VAF on 1.5B flow cell Run: https://ilmn-tsotest.basespace.illumina.com/s/wS4hjl9WD1Py Project: https://ilmn-tsotest.basespace.illumina.com/s/RU9IFRUTOckR TruSight Oncology 500 ctDNA v2; Cell line mixes samples on 10B flow cellRun: https://ilmn-tsotest.basespace.illumina.com/s/nwh4WgkNgpPg Project: https://ilmn-tsotest.basespace.illumina.com/s/RU9IFRUTOckR TruSight Oncology 500 ctDNA v2; 0.5% VAF SeraSeq ctDNA Complete Mutation Mix samples on 1.5B flow cell Run: https://ilmn-tsotest.basespace.illumina.com/s/WCK0QV7op8JjProject: https://ilmn-tsotest.basespace.illumina.com/s/RU9IFRUTOckR TruSight Oncology 500 ctDNA v2; 8 SeraSeq complete Mutation Mix samples on S2 flow cellRun: https://basespace.illumina.com/s/V506TtPbrkaDProject: https://basespace.illumina.com/s/H0O9C03OPbzg NextSeq 2000 TruSight Oncology ctDNA 500 V2 with Seracare Controls at 0.5% VAF Project: https://basespace.illumina.com/s/Ppzn6tay3AO3
SeraSeq® ctDNA Mutation Mix v2	TruSight Oncology 500 ctDNA v2; 4 SeraSeq Mutation Mix v2 samples on S2 flow cell Run: https://basespace.illumina.com/s/VWPmw2CfIR1JProject: https://basespace.illumina.com/s/v0x6nsUx9H3l

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