# Information on controls used in TruSight Oncology 500 demo datasets

**Background**

Illumina provides demo datasets for the TruSight Oncology 500 (TSO500) assay and analysis in the [Demo Data](https://basespace.illumina.com/datacentral) section of BaseSpace to allow customers to understand expected outputs and results. Each demo dataset contains commercially available control samples, with some also including internally-derived test samples. In the tables below demo datasets are listed along with the controls used in each.

**TSO500 (FFPE, solid assay)**

**TruSight Oncology 500; Commerical controls (DNA + RNA) on NextSeq 550**Project:<https://basespace.illumina.com/s/RfIEI4oB2ePi>

|                               |                 |                |                                                             |
| ----------------------------- | --------------- | -------------- | ----------------------------------------------------------- |
| **Sample ID**                 | **Sample Type** | **Vendor**     | **Item**                                                    |
| SeraCare0710\_0097            | DNA             | SeraCare       | Seraseq® Tri-Level Tumor Mutation DNA Mix v2                |
| HorizonDx\_HD827              | DNA             | HorizonDx      | HD827: Mimix™ OncoSpan, gDNA Reference Standard             |
| HorizonDx\_HD753              | DNA             | HorizonDx      | HD753: Mimix™ Structural Multiplex, FFPE Reference Standard |
| MilliporeSigma\_87060101\_1VL | DNA             | MilliporeSigma | LoVo Cell Line human                                        |
| ATCC\_HCC\_1395\_CRL\_2324D   | DNA             | ATCC           | HCC1395 CRL-2324 ™                                          |
| ATCC\_A2058\_CRL\_11147D      | DNA             | ATCC           | A2058 CRL-3601 ™                                            |
| ATC\_CCCL\_225DQ              | DNA             | ATCC           | HCT-15 Purified Genomic DNA, Quantitative (KRAS Mutant)     |
| RNA\_SeraCare\_0710\_0496     | RNA             | SeraCare       | Seraseq® FFPE Tumor Fusion RNA v4 Reference Material        |
| RNA\_SeraCare\_0710\_1031     | RNA             | SeraCare       | Seraseq® FFPE NTRK Fusion RNA Reference Material            |
| RNA\_SeraCare\_0710\_0137     | RNA             | SeraCare       | Seraseq® FFPE WT (DNA/RNA) Reference Material               |
| UHR\_740000                   | RNA             | Agilent        | Universal Human Reference RNA                               |

**NovaSeq 6000Dx: TruSight Oncology 500 v2**\
Project: <https://basespace.illumina.com/s/k5If7vXHZshI>

|                  |                 |            |                                                                 |
| ---------------- | --------------- | ---------- | --------------------------------------------------------------- |
| **Sample ID**    | **Sample Type** | **Vendor** | **Item**                                                        |
| HD795\_rep1\_dna | DNA             | HorizonDx  | HD795: Mimix™ BRCA Somatic Multiplex I, FFPE Reference Standard |

**TSO500 ctDNA (liquid assay)**

|                                      |                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              |
| ------------------------------------ | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ |
| **Control**                          | **Demo data**                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                |
| Seraseq® ctDNA Complete Mutation Mix | TruSight Oncology 500 ctDNA v2; SeraSeq ctDNA Complete Mutation Mix variants at 0.2% VAF on 1.5B flow cell Run: <https://ilmn-tsotest.basespace.illumina.com/s/wS4hjl9WD1Py> Project: <https://ilmn-tsotest.basespace.illumina.com/s/RU9IFRUTOckR> TruSight Oncology 500 ctDNA v2; Cell line mixes samples on 10B flow cellRun: <https://ilmn-tsotest.basespace.illumina.com/s/nwh4WgkNgpPg> Project: <https://ilmn-tsotest.basespace.illumina.com/s/RU9IFRUTOckR> TruSight Oncology 500 ctDNA v2; 0.5% VAF SeraSeq ctDNA Complete Mutation Mix samples on 1.5B flow cell Run: <https://ilmn-tsotest.basespace.illumina.com/s/WCK0QV7op8Jj>Project: <https://ilmn-tsotest.basespace.illumina.com/s/RU9IFRUTOckR> TruSight Oncology 500 ctDNA v2; 8 SeraSeq complete Mutation Mix samples on S2 flow cellRun: <https://basespace.illumina.com/s/V506TtPbrkaD>Project: <https://basespace.illumina.com/s/H0O9C03OPbzg> NextSeq 2000 TruSight Oncology ctDNA 500 V2 with Seracare Controls at 0.5% VAF Project: <https://basespace.illumina.com/s/Ppzn6tay3AO3> |
| SeraSeq® ctDNA Mutation Mix v2       | TruSight Oncology 500 ctDNA v2; 4 SeraSeq Mutation Mix v2 samples on S2 flow cell Run: <https://basespace.illumina.com/s/VWPmw2CfIR1J>Project: <https://basespace.illumina.com/s/v0x6nsUx9H3l>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               |

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