# Information on controls used in TruSight Oncology 500 demo datasets

**Background**

Illumina provides demo datasets for the TruSight Oncology 500 (TSO500) assay and analysis in the [Demo Data](https://basespace.illumina.com/datacentral) section of BaseSpace to allow customers to understand expected outputs and results. Each demo dataset contains commercially available control samples, with some also including internally-derived test samples. In the tables below demo datasets are listed along with the controls used in each.

**TSO500 (FFPE, solid assay)**

**TruSight Oncology 500; Commerical controls (DNA + RNA) on NextSeq 550**Project:<https://basespace.illumina.com/s/RfIEI4oB2ePi>

|                               |                 |                |                                                             |
| ----------------------------- | --------------- | -------------- | ----------------------------------------------------------- |
| **Sample ID**                 | **Sample Type** | **Vendor**     | **Item**                                                    |
| SeraCare0710\_0097            | DNA             | SeraCare       | Seraseq® Tri-Level Tumor Mutation DNA Mix v2                |
| HorizonDx\_HD827              | DNA             | HorizonDx      | HD827: Mimix™ OncoSpan, gDNA Reference Standard             |
| HorizonDx\_HD753              | DNA             | HorizonDx      | HD753: Mimix™ Structural Multiplex, FFPE Reference Standard |
| MilliporeSigma\_87060101\_1VL | DNA             | MilliporeSigma | LoVo Cell Line human                                        |
| ATCC\_HCC\_1395\_CRL\_2324D   | DNA             | ATCC           | HCC1395 CRL-2324 ™                                          |
| ATCC\_A2058\_CRL\_11147D      | DNA             | ATCC           | A2058 CRL-3601 ™                                            |
| ATC\_CCCL\_225DQ              | DNA             | ATCC           | HCT-15 Purified Genomic DNA, Quantitative (KRAS Mutant)     |
| RNA\_SeraCare\_0710\_0496     | RNA             | SeraCare       | Seraseq® FFPE Tumor Fusion RNA v4 Reference Material        |
| RNA\_SeraCare\_0710\_1031     | RNA             | SeraCare       | Seraseq® FFPE NTRK Fusion RNA Reference Material            |
| RNA\_SeraCare\_0710\_0137     | RNA             | SeraCare       | Seraseq® FFPE WT (DNA/RNA) Reference Material               |
| UHR\_740000                   | RNA             | Agilent        | Universal Human Reference RNA                               |

**NovaSeq 6000Dx: TruSight Oncology 500 v2**\
Project: <https://basespace.illumina.com/s/k5If7vXHZshI>

|                  |                 |            |                                                                 |
| ---------------- | --------------- | ---------- | --------------------------------------------------------------- |
| **Sample ID**    | **Sample Type** | **Vendor** | **Item**                                                        |
| HD795\_rep1\_dna | DNA             | HorizonDx  | HD795: Mimix™ BRCA Somatic Multiplex I, FFPE Reference Standard |

**TSO500 ctDNA (liquid assay)**

|                                      |                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              |
| ------------------------------------ | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ |
| **Control**                          | **Demo data**                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                |
| Seraseq® ctDNA Complete Mutation Mix | TruSight Oncology 500 ctDNA v2; SeraSeq ctDNA Complete Mutation Mix variants at 0.2% VAF on 1.5B flow cell Run: <https://ilmn-tsotest.basespace.illumina.com/s/wS4hjl9WD1Py> Project: <https://ilmn-tsotest.basespace.illumina.com/s/RU9IFRUTOckR> TruSight Oncology 500 ctDNA v2; Cell line mixes samples on 10B flow cellRun: <https://ilmn-tsotest.basespace.illumina.com/s/nwh4WgkNgpPg> Project: <https://ilmn-tsotest.basespace.illumina.com/s/RU9IFRUTOckR> TruSight Oncology 500 ctDNA v2; 0.5% VAF SeraSeq ctDNA Complete Mutation Mix samples on 1.5B flow cell Run: <https://ilmn-tsotest.basespace.illumina.com/s/WCK0QV7op8Jj>Project: <https://ilmn-tsotest.basespace.illumina.com/s/RU9IFRUTOckR> TruSight Oncology 500 ctDNA v2; 8 SeraSeq complete Mutation Mix samples on S2 flow cellRun: <https://basespace.illumina.com/s/V506TtPbrkaD>Project: <https://basespace.illumina.com/s/H0O9C03OPbzg> NextSeq 2000 TruSight Oncology ctDNA 500 V2 with Seracare Controls at 0.5% VAF Project: <https://basespace.illumina.com/s/Ppzn6tay3AO3> |
| SeraSeq® ctDNA Mutation Mix v2       | TruSight Oncology 500 ctDNA v2; 4 SeraSeq Mutation Mix v2 samples on S2 flow cell Run: <https://basespace.illumina.com/s/VWPmw2CfIR1J>Project: <https://basespace.illumina.com/s/v0x6nsUx9H3l>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               |

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                  |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #10057), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000010057%20-%20Software%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


---

# Agent Instructions: Querying This Documentation

If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://knowledge.illumina.com/software/tso500-tst170/software-tso500-tst170-reference_material-list/000010057.md?ask=<question>
```

The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.