> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/software/dragen/software-dragen-faq-list/000006031.md).

# How to enable DRAGEN to process multiple FASTQ files for the same subject?

**How to enable DRAGEN to process multiple FASTQ files, produced by the Illumina bcl2fastq or BCL Convert programs, for the same subject?**

The command line options, combine-samples-by-name or fastq-list, can be used for this purpose. Both options are specified in the User Guide. The fastq-list is the preferred option, which will let you specify all FASTQ files for a sample along with read group IDs. The fastq-list is in CSV format and can include all the samples in a flowcell/run (See the [FASTQ List documentation](https://help.dragen.illumina.com/product-guide/dragen-v4.4/bcl-conversion#fastq-list-output-file) for more information about FASTQ List format).

To process a specific sample from the CSV file, use the --fastq-list-sample-id {SampleID} option. Only the entries in the fastq-list file with an RGSM value matching the specified SampleID are processed. If using DRAGEN BCL conversion, then the conversion process will also generate these fastq-list CSV for you.

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                 |
| :-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #6031), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000006031%20-%20Software%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


---

# Agent Instructions
This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com.

## Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter, and the optional `goal` query parameter:

```
GET https://knowledge.illumina.com/software/dragen/software-dragen-faq-list/000006031.md?ask=<question>&goal=<endgoal>
```

`ask` is the immediate question: it should be specific, self-contained, and written in natural language.
`goal` is optional and describes the broader end goal you are ultimately trying to accomplish on behalf of the user. GitBook uses it to tailor the answer towards what is most useful for that goal.

The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.
