Troubleshooting DRAGEN Array Cloud error Sample sheet missing required columns

DRAGEN Array v1.3 introduced new sample sheet features:

• GenomeStudio backwards compatible samplesheet support and related deprecation of separate IDAT and GTC samplesheets.

• User-defined data from the samplesheet will get passed to gt_sample_summary files during genotyping.

These sample sheet features are compatible only with DRAGEN Array Local CLI Analysis.

When launching DRAGEN Array Cloud Analysis on BaseSpace using a GenomeStudio-formatted sample sheet, the following error appears: Samplesheet is missing required columns "beadChipName" and "sampleSectionName"

To resolve this error, use correct format for Cloud sample sheet. Cloud analysis does not currently support sections found the GenomeStudio sample sheet such as Header and Data. Cloud analysis does not use SentrixBarcode_A and SentrixPosition_A to identify samples.

DRAGEN Array Cloud sample sheet uses the following 2 column headers which must be included in the CSV file:

• beadChipName

• sampleSectionName

Example:

Cloud Sample Sheet Template is available on the sample selection interface on BaseSpace:

Local and Cloud Sample Sheet templates are available on the DRAGEN Array Secondary Analysis Software Downloads Page.

For more information, see DRAGEN Array User Guide and Release Notes.

For any feedback or questions regarding this article (Illumina Knowledge Article #10004), contact Illumina Technical Support [email protected].