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How to switch between AutoLoader Mode and Manual Mode when using iScan

When operating an Illumina iScan with an AutoLoader 2.x, users might need to switch between AutoLoader 2.x and Manual mode to use the iScan with or without the AutoLoader 2.x.

  • To run the iScan System without the AutoLoader 2.x, switch the reader into Manual mode.

  • To return to using AutoLoader 2.x, simply switch the readers back into AutoLoader mode.

Switching from AutoLoader 2.x Mode to Manual Mode

  1. Complete all automated scans.

  2. Remove all carriers from the AutoLoader 2.x input stacks.

  3. In the iScan Control Software (ICS), select the Menu button and select 'Exit'.

Figure 1. ICS menu with the 'Exit' button highlighted.

  1. Restart the iScan Control Software. The software then prompts the user to run the iScan system in AutoLoader mode or Manual mode.

Figure 2. ICS Scan Mode selection upon restarting the software.

  1. Select 'No' to run the iScan System in Manual mode. For information on manual scanning, refer to the iScan System Guide.

Switching from Manual Mode to AutoLoader 2.x Mode

  1. Complete all manual scans.

  2. Place the carriers to be scanned on the AutoLoader 2.x input stacks.

  3. To run the AutoLoader 2.x in dual-reader mode, make sure the iScan Control Software is closed on the remote reader.

  4. Follow the instructions for scanning BeadChips using an AutoLoader 2.x. Refer to section on Scan BeadChips with AutoLoader 2.x in the AutoLoader 2.x Product Documentation.

For any feedback or questions regarding this article (Illumina Knowledge Article #5545), contact Illumina Technical Support techsupport@illumina.com.

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