BlueFuse Multi: How to mark regions as "Not Reported"

The administrator can set global algorithm settings to mark regions smaller than the default (eg. 50 kb), as not reported by default. Thus, when an experiment is processed, the flags are set to all regions smaller than this size as "Not Reported". A user can also configure this setting on an experiment-by-experiment basis and on a region-by-region basis during the analysis. Conversely, any region previously marked as "Not Reported" can be moved back to "Reported" and toggled between the two. This functionality facilitates regions being flagged "Reported" and "Not Reported" during the analysis and annotation of the test.

The ability to mark a region as reported/not reported is available for the following menu options:

Menu Option

Procedure

Description

CGH/CNV Table

Right-click the selection, and then select Mark Reported/Not Reported. The region is then marked "no" in the reported column and "yes" in the manual change column.

Allows you to select a region to include or remove from the report. If a region is marked as "not reported," the imbalance is removed from the report and no longer displayed in the Karyotype Chart (Reported). This change is recorded in the manual change column.

Decision Track

Right-click the selection, and then select Mark Reported/Not Reported. The region is then marked "no" in the reported column and "yes" in the manual change column.

Available when a region is selected. Allows you to select a region to include or remove from the report. If a region is marked as "not reported," the imbalance is removed from the report and no longer displayed in the Karyotype Chart (Reported). This change is recorded in the manual change column.

Region View (Detailed Region tab)

Remove a region using the dropdown menu. The region is then marked "no" in the reported column and "yes" in the manual change column.

Allows you to set genes as reported or not reported.

Region View (Reported Genes tab)

Right-click the selection, and then select Mark Reported/Not Reported. The region is then marked "no" in the reported column and "yes" in the manual change column.

Allows you to set genes as reported or not reported. Genes are removed from the Reported Genes tab.

After regions have been marked as Not Reported, they are excluded from the report, Karyotype Chart (Reported), CGH Chart (Reported), and CNV Chart (Reported).

For additional information, see BlueFuse Multi Software Guidearrow-up-right.

For any feedback or questions regarding this article (Illumina Knowledge Article #5042), contact Illumina Technical Support [email protected]envelope.

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