> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/microarray/general/microarray-general-reference_material-list/000002889.md).
# Infinium HTS BeadChip Hybridization Loading Patterns for Manual and Automated Protocols
The following guidance outlines the Infinium High-Throughput Screening (HTS) BeadChip loading patterns for both the manual workflow and the automated workflow using the Tecan/Infinium Automated Pipetting System (IAPS) with Illumina Automation Control (IAC). Understanding the differences between these loading patterns is important for ensuring correct sample placement on BeadChips and assist with troubleshooting by determining whether observed failure patterns correlate with specific sample plate positions.# **Infinium HTS Manual Protocol**
**Single-Channel Pipette**
Using a single-channel pipette, transfer 14 µl of each sample from the MSA3 plate to the appropriate section of the BeadChip, as shown in Figure 1.
Ensure that the pipette tips are properly positioned within the sample inlets before dispensing the samples onto the BeadChip to prevent loading errors and ensure accurate sample distribution.
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**Figure 1.** MSA3 plate layout (top) for the Infinium HTS manual protocol using a single-channel pipette, and the corresponding BeadChip loading pattern (bottom).
### **Multichannel Pipette**
Using an adjustable-spacing multichannel precision pipette, transfer 14 µl of each sample from the MSA3 plate to the appropriate section of the BeadChip, as shown in Figure 2.
Ensure that the pipette tips are properly positioned within the sample inlets before dispensing the samples onto the BeadChip to prevent loading errors and ensure accurate sample distribution.
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**Figure 2.** MSA3 plate layout (top) for the Infinium HTS manual protocol and using a multichannel pipette, and the corresponding BeadChip loading pattern (bottom).
## **Infinium HTS Automated Protocol (Tecan/IAPS with IAC)**
Refer to Figure 3 for the BeadChip loading pattern used in the automated workflow.
**Figure 3.** MSA3 plate layout (top) for the Infinium HTS automated protocol using the Tecan/IAPS with IAC, and the corresponding BeadChip loading pattern (bottom).
**References:**
* [Infinium HTS Assay Manual Lab Tracking Form](https://support.illumina.com/downloads/infinium_hts_assay_manual_protocol_lab_tracking_form_15047409_a.html)
* [Infinium HTS Automated Lab Tracking Form](https://support.illumina.com/downloads/infinium_hts_assay_automated_protocol_lab_tracking_form_15047410_a.html)
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