# Infinium HTS BeadChip Hyb loading pattern for manual vs automated protocol

#### **INFINIUM HTS MANUAL PROTOCOL**

**Single Channel Pipette**

Transfer 14 µl of each sample from the MSA3 plate to the appropriate section of the BeadChip.\
Make sure that the pipette tip is in the sample inlet before dispensing.

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-31c92d4f1b6205baf98468eb447a41cf7a7c9f83%2Fimage1.png?alt=media)

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-ad21cca4aa503b91a8f670e3a72b72411012d3dd%2Fimage2.png?alt=media)\
![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-23eb2f0addaa972e87e0387cc2f3250dfdf8a3cb%2Fimage3.png?alt=media)

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**Multichannel Pipette**

Using an adjustable spacer multichannel precision pipette, transfer 14 µl of each sample from the MSA3 plate to the appropriate section of the BeadChip.\
Make sure that the pipette tip is in the sample inlet before dispensing.

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-571a555620e63795957ff13bfb455a59642490b1%2Fimage4.png?alt=media)

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-23f9d999f32a0e289718520e349970ccfb2a07ff%2Fimage5.png?alt=media)\
![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-cff65f9c3eb6db7879229213456f6cd82ab38d8b%2Fimage6.png?alt=media)

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#### **INFINIUM HTS AUTOMATED PROTOCOL (TECAN/IAPS WITH IAC)**

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-085a702b301d53cd46d8381a7594d0ae614647be%2Fimage7.png?alt=media)

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-1f161edd59fc2164d643422fa110711045fd559d%2Fimage8.png?alt=media)

**For additional information, see the following:**

* [Infinium HTS Assay Manual Lab Tracking Form](https://support.illumina.com/downloads/infinium_hts_assay_manual_protocol_lab_tracking_form_15047409_a.html)
* [Infinium HTS Automated Lab Tracking Form](https://support.illumina.com/downloads/infinium_hts_assay_automated_protocol_lab_tracking_form_15047410_a.html)
* [Infinium HD Assay Ultra Protocol Guide](https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/infinium_assays/infinium-hd-ultra/11328087_RevB_Infinium_HD_Ultra_Assay_Guide_press.pdf)

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #2889), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000002889%20-%20Microarray%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |