What Is the Expected DNA Fragment Size Following the Fragment DNA Step of the Infinium Assay?

In the Infinium Assay workflow, addition of the FMS (fragmentation solution) reagent to the MSA plate results in endpoint fragmentation of the amplified DNA. This controlled enzymatic process generates DNA fragments approximately 300-600 base pairs (bp) in length, which are optimal for hybridization to the Infinium array. References:

• Infinium Chemistry Online Training

• Infinium Assay Workflow Technical Note

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