General information regarding RNA samples in the TruSight Oncology 500 (TSO500) v1 and v2 workflows

What is the protocol for preparing RNA samples in the TSO500 workflow? Refer to the TSO500 v1 reference guide or the TSO500 v2 product documentation for the protocol; both the DNA and RNA protocols are included in the guides.

Are the Unique molecular identifier (UMI) adapters also used for RNA samples? In the v1 workflow, no, they are not; UMI adapters are used for DNA samples only. In the v2 workflow, the UMI adapters are used for both DNA and RNA samples.

Is the RNA content in the TSO500 DNA/RNA the same as the TruSight Tumor 170 (TST170) RNA content? Yes, TSO500 uses the TST170 RNA probe panel, OPR1.

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