> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/trusight-oncology-tumor-library-prep/library-preparation-trusight-oncology-tumor-library-prep-reference_material-list/000003171.md).

# General information regarding RNA samples in the TruSight Oncology 500 (TSO500) v1 and v2 workflows

**What is the protocol for preparing RNA samples in the TSO500 workflow?**\
Refer to the [TSO500 v1 reference guide](https://support.illumina.com/downloads/trusight-oncology-500-reference-guide-1000000067621.html) or the [TSO500 v2 product documentation](https://support.illumina.com/sequencing/sequencing_kits/trusight-oncology-500-v2/documentation.html) for the protocol; both the DNA and RNA protocols are included in the guides.

**Are the Unique molecular identifier (UMI) adapters also used for RNA samples?**\
In the v1 workflow, no, they are not; UMI adapters are used for DNA samples only. In the v2 workflow, the UMI adapters are used for both DNA and RNA samples.

**Is the RNA content in the TSO500 DNA/RNA the same as the TruSight Tumor 170 (TST170) RNA content?**\
Yes, TSO500 uses the TST170 RNA probe panel, OPR1.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #3171), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003171%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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