> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/rna-enrichment-based-library-prep/library-preparation-rna-enrichment-based-library-prep-faq-list/000003103.md).

# Illumina RNA Prep with Enrichment, (L) Tagmentation General FAQs

**What** **is Illumina RNA Prep with Enrichment, (L) Tagmentation Kit ?**

The Illumina RNA Prep with Enrichment, (L) Tagmentation kit generates targeted RNA libraries. The workflow is enrichment-based, meaning it uses oligo probes to pull-down regions of interest for sequencing.

**Why use a targeted RNA workflow instead of total RNA or mRNA sequencing?**

A targeted approach allows for sequencing a specific set of RNA transcripts. This reduces the number of required reads per sample and focuses the data on genes of interest.

**Which reagents are needed to order to complete the workflow?**

The [reference guide](https://support.illumina.com/downloads/illumina-rna-prep-reference-guide-1000000124435.html) lists the required Illumina kits and user-supplied consumables that are required for this application.

**Which RNA molecules are targeted with this kit?**

The kit selectively captures the regions for which the capture probes have been designed to enrich.

**What RNA molecules are depleted with this kit?**

There are no rRNA depletion or mRNA capture steps at the beginning of the library prep. Unwanted RNAs are not targeted for enrichment.

**Are the libraries stranded?**

The libraries are not stranded.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #3103), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003103%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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