> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/multiomics-library-prep/library-preparation-multiomics-library-prep-faq-list/000009947.md).

# Enrichment workflow FAQ for the Illumina 5 Base DNA Prep with Enrichment kit

**Are non-human or non-mouse (eg, zebrafish) samples supported?**

* Only human and mouse enrichment are supported with this product.

**For the Enrichment workflow, are there additional PCR cycles required?**

* Yes, additional PCR cycles are performed during the Enrichment workflow. See the Product Documentation for details.

**What plexity of enrichments are supported?**

* 4-plex. 2 µl of each of 4 libraries are pooled together in one enrichment reaction. Libraries are pooled by volume (not mass).

**Can pooling be done by mass or volume?**

* Pooling is done by volume, 2 ul per sample, into a 4-plex reaction for the hybridization.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #9947), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000009947%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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