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Best practices for pipette mixing PIPs and cells/nuclei in Illumina Single Cell 3' RNA Prep
During the Capture and Lysis portion of the Illumina Single Cell Prep workflow, users pipette mix the sample, RNase inhibitors, and PIPs before the addition of partitioning reagent and droplet formation.
It is important to avoid creating foam; mix the viscous cell:PIP mixture slowly and do not press the pipette to the second stop. If excessive foam forms, centrifuge the PIP tubes on a minifuge for ~5 seconds and repeat pipette mixing.
For any feedback or questions regarding this article (Illumina Knowledge Article #9709), contact Illumina Technical Support techsupport@illumina.com.
