Representative library QC traces for Illumina Single Cell 3’ RNA Prep

The final Illumina Single Cell 3' RNA Prep library distributions and average fragment sizes may vary based on sample type and experimental conditions.

The following TapeStation traces serve as representative examples of satisfactory library QC profiles (different from satisfactory cDNA QC) generated during the Check Library step of the Illumina Single Cell 3' RNA Prep workflow. For more information, see the appropriate product documentation (protocol).

Figure 1. Human peripheral blood mononuclear cells (PBMCs)

Figure 2. Human HEK 293T and mouse NIH 3T3 cell line mixture

Figure 3. Mouse frozen tissue: unfixed brain nuclei

Figure 4. Mouse frozen tissue: DSP-methanol fixed brain nuclei

Figure 5. Mouse frozen tissue: unfixed lung nuclei

For more information related to library QC product assessment refer to:

• Assessing library QC in Illumina Single Cell 3' RNA Prep

• Illumina Single Cell 3' RNA Prep library QC best practices

• Illumina Single Cell 3' RNA Prep Training Packet

For any feedback or questions regarding this article (Illumina Knowledge Article #9528), contact Illumina Technical Support [e-mail].