> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000009958.md).

# Illumina library applications for 2x500 bp sequencing on the MiSeq i100

Illumina launched the [MiSeq i100 Series 25M Reagent Kit (1000 cycles)](https://www.illumina.com/products/by-type/sequencing-kits/cluster-gen-sequencing-reagents/miseq-i100-series-reagent-kits.html#tabgroup-1-tab2) on October 20, 2025, to enable 2x501 bp sequencing on the MiSeq i100. Illumina has tested modifications to existing library preparation workflows to increase library sizing to leverage these longer read lengths.

See the following for resources for longer insert library preparation. Additional resources for longer insert library preparation will be linked within this article as they become available.

* [Illumina DNA Prep library preparation for the MiSeq i100 Series 25M Reagent Kit (1000 cycles)](/library-preparation/general/library-preparation-general-reference_material-list/000010102.md)
* [16S library preparation for the MiSeq i100 Series 25M Reagent Kit (1000 cycles)](/library-preparation/amplicon-library-prep/library-preparation-amplicon-library-prep-reference_material-list/000010152.md)

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #9958), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000009958%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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