Respiratory Virus Enrichment Kit sequencing recommendations and public data resources

What is the recommended read length? A 2x75 bp read length is recommended when sequencing Respiratory Virus Enrichment Kit (RVEK) libraries, prepared with the Respiratory Virus Oligos Panel v2 (RVOP v2). This shorter read length takes into account the potentially shorter average size of libraries created with samples of variable quality.

What is the recommended read depth? 1 million reads per sample are recommended as a starting point, labs can adjust based on data needs.

Are there public datasets available? Yes, the following public datasets are available in BaseSpace Sequence Hub. (Note: BaseSpace login is required to access the following links.)

iSeq 100: Illumina RNA Prep with Enrichment and RVOPv2 (SARS-CoV-2 Synthetic RNA Control) Run Link Project Link

MiniSeq: Illumina RNA Prep with Enrichment and RVOPv2 (SARS-CoV-2 Synthetic RNA Control) Run Link Project Link

MiSeq: Illumina RNA Prep with Enrichment and RVOPv2 (SARS-CoV-2 Synthetic RNA Control) Run Link Project Link

NextSeq 550: Illumina RNA Prep with Enrichment and RVOPv2 (SARS-CoV-2 Synthetic RNA Control). Run Link Project Link

Please note that this kit was previously named the Respiratory Virus Oligo Panel v2 kit, and has been renamed to the Respiratory Virus Enrichment Kit, which still uses the Respiratory Virus Oligo Panel v2 for enrichment.

For any feedback or questions regarding this article (Illumina Knowledge Article #3426), contact Illumina Technical Support [e-mail].