> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000003305.md).

# Best practices for handling magnetic beads used with Illumina RNA library preparation kits

**Best practices for handling magnetic beads include the following. Some recommendations are only relevant to certain bead types used in library preparation.**

* Bring beads to room temperature more than 30 minutes before use
* Verify use of correct bead type/tube
* Vortex beads thoroughly to fully resuspend
* Use recommended plates and magnetic stand
* Carefully pipette up and down to minimize sample loss
* Use freshly prepared 80% ethanol, when called for
* Fully remove/dry ethanol prior to subsequent steps; residual ethanol may interfere with downstream steps
* Do not allow beads to over dry
* Do not freeze beads which require 4°C storage; always store beads according to official recommendations

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #3305), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003305%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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