> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000010212.md).

# Input recommendations for Illumina Rapid RNA Kits

**Are there any changes to the RNA input quantity or quality that the Rapid RNA kits support vs non-Rapid?**

Yes, Illumina Rapid mRNA and Illumina Rapid RNA Prep with Enrichment workflows support lower input amounts (down to 1 ng of high quality input) compared to their non-Rapid versions. For traditional RNA-seq workflows, clean-up steps following cDNA synthesis or ligation may lose a significant amount of the sample library. Illumina Rapid workflows eliminate those clean-up steps, resulting in more diverse and complex mRNA libraries for detection of low abundance transcripts and supporting lower input amounts.

**Note**: Illumina Stranded Total RNA already supports down to 1 ng of input, therefore the lower end of the input recommendation for Illumina Rapid Total RNA is still also 1 ng.

The required input quality (ie, RIN or DV200) are unchanged compared to the non-Rapid versions. See the respective Product Documentation RNA Input Recommendations section for input recommendation details for each Illumina Rapid RNA product.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #10212), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000010212%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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