> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006988.md).
# How to pool Illumina DNA Prep with Exome 2.5 libraries on the NextSeq series and NovaSeq 6000?
Recommendations for approximate number of samples to pool for estimated coverages of 50X, 100X, or 200X, for the NextSeq 500/550, NextSeq 1000/2000, and NovaSeq 6000 are available in the Illumina DNA Prep with Exome 2.5 Enrichment [Data Sheet](https://support.illumina.com/content/dam/illumina/gcs/assembled-assets/marketing-literature/illumina-dna-exome-enrich-m-gl-00002/illumina-dna-exome-enrich-data-sheet-m-gl-00002.pdf).
These are estimates and each lab must verify empirically. Also, note that the reference guide does not contain specific instructions for plexities between 1-plex and 12-plex, so this may require optimization. Illumina does not have specific guidance for other sequencing instruments.
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| *For any feedback or questions regarding this article (Illumina Knowledge Article #6988), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000006988%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |
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