> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005793.md).

# Are the Illumina RNA Index Anchors the same in every well of the plate?

The Illumina RNA Index Anchors plate (reference number 20040899) is included with the Illumina RNA UD Indexes, Ligation (96 Indexes, 96 Samples) (reference numbers 20091655 (Set A), 20091657 (Set B), 20091659 (Set C), 20091661 (Set D)) for use with Illumina Stranded Total RNA Prep and Illumina Stranded mRNA Prep Kits.

* Each well of the anchor plate is single-use and contains RNA Index Anchors.
* All anchor plate wells contain the same reagent and can be used in any order. (All the wells are the same.)
* The well positions used in the RNA Index Anchor plate do not need to match the index primer well positions used in the index plate.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #5793), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000005793%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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