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Converting ng/µl to nM when calculating dsDNA library concentration
Many standard Illumina libraries, such as Illumina DNA Prep, require the use of dsDNA-specific fluorescent dye methods for accurate quantification. These methods typically measure dsDNA concentration in ng/µl. To convert from ng/µl to nM for cluster generation, follow the instructions below. 1. Determine the average size of the library by running it on an Agilent Technologies TapeStation, 2100 Bioanalyzer, or Fragment Analyzer.
Use the following formula to convert from ng/μl to nM:
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