Reference Material

Library Preparation > General Library Prep > Reference Material

• Adapter dimers causes, effects, and how to remove them

• Bead types in Illumina library preparation kits

• Best practices for library quantification

• Best practices for manually normalizing library concentrations

• Bubble products in sequencing libraries: causes, identification, and workflow recommendations

• Converting ng/µl to nM when calculating dsDNA library concentration

• DNA/RNA isolation considerations when using Illumina library prep kits

• Double sided size selection and bead clean up

• How to Plan Your First Sequencing Project Support Webinar Video

• How to mitigate index hopping

• How to view more content and products relevant to specific fields of interest on the Illumina website

• IDT for Illumina DNA/RNA Unique Dual Index Compatibility on the MiniSeq

• Is it possible to pool different library types in the same sequencing run?

• Library Prep Bead Handling Workflows Best Practices LNB1/2/3 (Video)

• Library Prep Bead Handling Workflows Best Practices SMB (Video)

• Library Prep Bead Handling Workflows Best Practices SPB/IPB Video

• Library quantification and quality control quick reference guide

• Plate layout for the Illumina DNA/RNA UD Indexes (v3)

• Sequencing FFPE Samples Introduction Support Webinar Video

• Understanding the differences between Illumina index chemistries and anchor function

• What is the PhiX Control v3 Library and what is its function in Illumina Next Generation Sequencing