> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/general-library-prep.md).

# General Library Prep

- [Troubleshooting](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-troubleshooting-list.md)
- [Troubleshooting elevated PhiX alignment in sequencing runs](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-troubleshooting-list/000009236.md)
- [FAQ](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-faq-list.md)
- [Can library preparation reagents be stored in a frost free freezer?](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-faq-list/000005798.md)
- [How to perform library heat denaturation (heat shock) before sequencing?](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-faq-list/000005129.md)
- [How to prepare < 2 nM libraries for sequencing on NovaSeq X/X Plus](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-faq-list/000009000.md)
- [Indexed Sequencing workflows with 5' to 3' directionality](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-faq-list/000005679.md)
- [What are the Illumina sequencing primer sequences?](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-faq-list/000007129.md)
- [Where to find the reference sequence used for phiX alignment?](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-faq-list/000005582.md)
- [Reference Material](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list.md)
- [Adapter dimers causes, effects, and how to remove them](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001911.md)
- [Bead types in Illumina library preparation kits](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000003342.md)
- [Best practices for library quantification](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000003750.md)
- [Best practices for manually normalizing library concentrations](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001252.md)
- [Bubble products in sequencing libraries: causes, identification, and workflow recommendations](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001918.md)
- [Converting ng/µl to nM when calculating dsDNA library concentration](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001240.md)
- [DNA/RNA isolation considerations when using Illumina library prep kits](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001249.md)
- [Double sided size selection and bead clean up](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000006157.md)
- [How to Plan Your First Sequencing Project Support Webinar Video](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000008042.md)
- [How to mitigate index hopping](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001928.md)
- [How to view more content and products relevant to specific fields of interest on the Illumina website](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000002704.md)
- [IDT for Illumina DNA/RNA Unique Dual Index Compatibility on the MiniSeq](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000002045.md)
- [Is it possible to pool different library types in the same sequencing run?](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000003284.md)
- [Library quantification and quality control quick reference guide](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001247.md)
- [Understanding the differences between Illumina index chemistries and anchor function](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000006561.md)
- [What is the PhiX Control v3 Library and what is its function in Illumina Next Generation Sequencing](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001545.md)


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