> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/general-library-prep.md).

# General Library Prep

- [FAQ](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-faq-list.md)
- [Can library preparation reagents be stored in a frost-free freezer?](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-faq-list/000005798.md)
- [How to perform library heat denaturation (heat shock) before sequencing?](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-faq-list/000005129.md)
- [How to prepare < 2 nM libraries for sequencing on NovaSeq X/X Plus](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-faq-list/000009000.md)
- [What are Single Reads (SR), Paired-End (PE) reads, and read requirements for sequencing?](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-faq-list/000009547.md)
- [What are the Illumina sequencing primer sequences?](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-faq-list/000007129.md)
- [Where to find the reference sequence used for PhiX alignment?](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-faq-list/000005582.md)
- [Reference Material](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list.md)
- [Adapter dimers causes, effects, and how to remove them](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001911.md)
- [Bead types in Illumina library preparation kits](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000003342.md)
- [Best practices for library quantification](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000003750.md)
- [Best practices for manually normalizing library concentrations](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001252.md)
- [Bubble products in sequencing libraries: causes, identification, and workflow recommendations](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001918.md)
- [Converting ng/µl to nM when calculating dsDNA library concentration](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001240.md)
- [DNA/RNA isolation considerations when using Illumina library prep kits](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001249.md)
- [Double-sided size selection and bead clean-up](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000006157.md)
- [How to Plan Your First Sequencing Project Support Webinar Video](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000008042.md)
- [How to mitigate index hopping](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001928.md)
- [How to view more content and products relevant to specific fields of interest on the Illumina website](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000002704.md)
- [Is it possible to pool different library types in the same sequencing run?](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000003284.md)
- [Library Prep Bead Handling Workflows Best Practices - LNB1/2/3 (Video)](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000010037.md)
- [Library Prep Bead Handling Workflows Best Practices - SMB (Video)](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000010038.md)
- [Library Prep Bead Handling Workflows Best Practices - SPB/IPB Video](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000010040.md)
- [Library quantification and quality control quick reference guide](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001247.md)
- [Metagenomics Part I Introduction to Library Preparation and Sequencing Support Webinar Video](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000008057.md)
- [Optimal Cluster Density Best Practices Video](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000005778.md)
- [Plate layout for the Illumina DNA/RNA UD Indexes (v3)](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000008364.md)
- [Resources for getting started with Illumina Next Generation Sequencing (NGS)](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000002171.md)
- [Sequencing FFPE Samples Introduction Support Webinar Video](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000008050.md)
- [Understanding the differences between Illumina index chemistries and anchor function](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000006561.md)
- [Using Sequencing Method Explorer to identify suitable NGS methods for research](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001245.md)
- [What is the PhiX Control v3 Library and what is its function in Illumina Next Generation Sequencing](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-reference_material-list/000001545.md)
- [Troubleshooting](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-troubleshooting-list.md)
- [Library QC and Troubleshooting with the Bioanalyzer and Fragment Analyzer Support Webinar Video](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-troubleshooting-list/000008041.md)
- [Preventing Contamination - Expert Video Tips](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-troubleshooting-list/000005747.md)
- [Troubleshooting elevated PhiX alignment in sequencing runs](https://knowledge.illumina.com/library-preparation/general-library-prep/library-preparation-general-library-prep-troubleshooting-list/000009236.md)


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