> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/dna-enrichment-based-library-prep/library-preparation-dna-enrichment-based-library-prep-faq-list/000009491.md).

# Workflow FAQ for Illumina FFPE DNA Prep with Exome 2.5 Enrichment

\*\*Does this kit use tagmentation?\*\*No, input DNA is sheared by Covaris. DNA is then end-repaired, A-tailed, and adapters are ligated and amplified prior to enrichment with the Twist Bioscience for Illumina Exome 2.5 panel. Libraries go through a final amplification and cleanup prior to sequencing.

\*\*Is automation supported? If yes, how many samples can be processed per kit? Are there any Illumina Ready or known vendor-developed methods?\*\*This kit is automation friendly. The kit name contains the number of samples that can be processed by automation. For example, the Illumina Cell-Free and FFPE DNA Prep, Ligation (16 Samples) (catalog 20104105) kit can process 16 samples by automation, or 24 samples manually. The number of samples that can be processed manually is contained in the "i" information dropdown on the product page.

There are no supported automation methods for Illumina FFPE DNA Prep with Exome 2.5 Enrichment kit at this time; Illumina is only providing automation-compatible reagents.

\*\*What are the supported plexities for enrichment?\*\*Illumina only supports 4-plex enrichment with Illumina FFPE DNA Prep with Exome 2.5 Enrichment workflow.

\*\*Does the FFPE workflow also support the Twist Bioscience for Illumina Mitochondrial panel or Illumina Custom Enrichment Panel v2 designed via DesignStudio?\*\*Yes, both supplemental enrichments are supported. Follow the guidance in the Supplemental Enrichment (Optional) section of the [protocol](https://sapac.support.illumina.com/downloads/ilmn-ffpe-dna-prep-exome-enrichment-product-documentation.html).

**What are typical pre-enrichment library yields and final library yields?**

* Pre-enrichment library yield is expected to be > 150 ng/µl
* Final library yield is expected to be > 3 ng/µl

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #9491), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000009491%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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