> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/dna-enrichment-based-library-prep/library-preparation-dna-enrichment-based-library-prep-faq-list/000009490.md).

# Input FAQ for Illumina FFPE DNA Prep with Exome 2.5 Enrichment

\*\*How should input be quantified and qualified?\*\*Quantification should be performed with a fluorometric quantification method such as Qubit, while qualification should be performed with the new TruSight FFPE QC kit. Use DNA samples that result in delta Cq (dCq) of < 4.

\*\*Is there any guidance for using DIN or DV200 instead of the TruSight FFPE QC kit?\*\*No, samples must be qualified by the TruSight FFPE QC kit.

\*\*Why is it necessary to shear at all when the input is already degraded?\*\*It is for consistency to make sure all DNA is fragmented into pieces that can be incorporated into libraries.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #9490), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000009490%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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