Troubleshooting a dry system thermal pre-run check failure on the NovaSeq X Series

Background

For NovaSeq X Series instruments, a pre-run check is performed prior to sequencing. During this process, the thermal check in the dry check may occasionally result in a failure. In such cases, a message similar to the one shown below may be displayed on the control software.

TemperatureAirxxx with value ## DegC was greater than ## DegC.

Troubleshooting steps

1. Check the laboratory environment.

   • Verify the room temperature. If it is higher than the acceptable range, request that it be lowered.

   • Check the filter condition:

     • Make sure the filter has been replaced appropriately.

     • Confirm that the filter is not blocked or clogged.

2. Retry the Pre-Run Check, by selecting Retry.

3. If the error occurs again:

   • • Power cycle the instrument.

     • Reconfigure and start the run again.

4. If the issue persists, contact Illumina Technical Support.

For any feedback or questions regarding this article (Illumina Knowledge Article #10228), contact Illumina Technical Support [e-mail].